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Literature summary for 3.4.21.100 extracted from
- Sedolisins, a new class of secreted proteases from Aspergillus fumigatus with endoprotease or tripeptidyl-peptidase activity at acidic pHs (2006), Appl. Environ. Microbiol., 72, 1739-1748.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | potential importance as a virulence factor and in the industrial hydrolysis of protein source | Aspergillus fumigatus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| SedA and SedC cloned into vector pUC18. Heterologously expressed in Pichia pastoris. SedA, SedB, SedC and SedD cloned into vector pET-11aH6 and expressed in Escherichia coli BL21 | Aspergillus fumigatus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | sedB-, sedC-, and sedD-negative mutants have 20%, 90%, and 80% of the tripeptidyl-peptidase activity of wild-type. None of the sed-negative mutants have a visible phenotype on Sabouraud agar or in hemoglobin liquid medium | Aspergillus fumigatus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 46000 | - |
x * 479000, mature sedA, sequence analysis. x * 430000, mature sedB, sequence analysis. x * 406000, mature sedD, sequence analysis. x * 55000, native sedA, SDS-PAGE. x * 60000-90000, native sedB, SDS-PAGE. x * 65000, native sedC, SDS-PAGE. x * 70000-100000, native sedD, SDS-PAGE. x * 46000, sedA and sedB after deglycosylation, SDS-PAGE. x * 48000, sedC and sedD after deglycosylation, SDS-PAGE | Aspergillus fumigatus |
| 48000 | - |
x * 479000, mature sedA, sequence analysis. x * 430000, mature sedB, sequence analysis. x * 406000, mature sedD, sequence analysis. x * 55000, native sedA, SDS-PAGE. x * 60000-90000, native sedB, SDS-PAGE. x * 65000, native sedC, SDS-PAGE. x * 70000-100000, native sedD, SDS-PAGE. x * 46000, sedA and sedB after deglycosylation, SDS-PAGE. x * 48000, sedC and sedD after deglycosylation, SDS-PAGE | Aspergillus fumigatus |
| 55000 | - |
x * 479000, mature sedA, sequence analysis. x * 430000, mature sedB, sequence analysis. x * 406000, mature sedD, sequence analysis. x * 55000, native sedA, SDS-PAGE. x * 60000-90000, native sedB, SDS-PAGE. x * 65000, native sedC, SDS-PAGE. x * 70000-100000, native sedD, SDS-PAGE. x * 46000, sedA and sedB after deglycosylation, SDS-PAGE. x * 48000, sedC and sedD after deglycosylation, SDS-PAGE | Aspergillus fumigatus |
| 65000 | - |
x * 479000, mature sedA, sequence analysis. x * 430000, mature sedB, sequence analysis. x * 406000, mature sedD, sequence analysis. x * 55000, native sedA, SDS-PAGE. x * 60000-90000, native sedB, SDS-PAGE. x * 65000, native sedC, SDS-PAGE. x * 70000-100000, native sedD, SDS-PAGE. x * 46000, sedA and sedB after deglycosylation, SDS-PAGE. x * 48000, sedC and sedD after deglycosylation, SDS-PAGE | Aspergillus fumigatus |
| 406000 | - |
x * 479000, mature sedA, sequence analysis. x * 430000, mature sedB, sequence analysis. x * 406000, mature sedD, sequence analysis. x * 55000, native sedA, SDS-PAGE. x * 60000-90000, native sedB, SDS-PAGE. x * 65000, native sedC, SDS-PAGE. x * 70000-100000, native sedD, SDS-PAGE. x * 46000, sedA and sedB after deglycosylation, SDS-PAGE. x * 48000, sedC and sedD after deglycosylation, SDS-PAGE | Aspergillus fumigatus |
| 430000 | - |
x * 479000, mature sedA, sequence analysis. x * 430000, mature sedB, sequence analysis. x * 406000, mature sedD, sequence analysis. x * 55000, native sedA, SDS-PAGE. x * 60000-90000, native sedB, SDS-PAGE. x * 65000, native sedC, SDS-PAGE. x * 70000-100000, native sedD, SDS-PAGE. x * 46000, sedA and sedB after deglycosylation, SDS-PAGE. x * 48000, sedC and sedD after deglycosylation, SDS-PAGE | Aspergillus fumigatus |
| 479000 | - |
x * 479000, mature sedA, sequence analysis. x * 430000, mature sedB, sequence analysis. x * 406000, mature sedD, sequence analysis. x * 55000, native sedA, SDS-PAGE. x * 60000-90000, native sedB, SDS-PAGE. x * 65000, native sedC, SDS-PAGE. x * 70000-100000, native sedD, SDS-PAGE. x * 46000, sedA and sedB after deglycosylation, SDS-PAGE. x * 48000, sedC and sedD after deglycosylation, SDS-PAGE | Aspergillus fumigatus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus fumigatus | - |
- |
- |
| Aspergillus fumigatus NCPF 7367 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| SedB purified by ion-exchange chromatography and gel filtration | Aspergillus fumigatus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | SedB, SedC, and SedD in culture supernatants grown in a medium containing hemoglobin as the sole nitrogen source | Aspergillus fumigatus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Ala-Ala-Phe-p-nitroanilide + H2O | SedB, SedC, and SedD | Aspergillus fumigatus | Ala-Ala-Phe + p-nitroaniline | - |
? |  |
| Ala-Ala-Phe-p-nitroanilide + H2O | SedB, SedC, and SedD | Aspergillus fumigatus NCPF 7367 | Ala-Ala-Phe + p-nitroaniline | - |
? | |
| Ala-Pro-Gly-Asp-Arg-Ile-Tyr-Val-His-Pro-Phe + H2O | SedB | Aspergillus fumigatus | Arg-Pro-Gly + Asp-Arg-Ile + Tyr-Val-His-Pro-Phe | - |
? | |
| Ala-Pro-Gly-Asp-Arg-Ile-Tyr-Val-His-Pro-Phe + H2O | SedB | Aspergillus fumigatus NCPF 7367 | Arg-Pro-Gly + Asp-Arg-Ile + Tyr-Val-His-Pro-Phe | - |
? | |
| additional information | SedA is an acidic endoprotease. SedB, SedC, and SedD are tripeptidyl-peptidases with a common specificity for tripeptide-p-nitroanilide substrates at acidic pHs | Aspergillus fumigatus | ? | - |
? | |
| additional information | SedA is an acidic endoprotease. SedB, SedC, and SedD are tripeptidyl-peptidases with a common specificity for tripeptide-p-nitroanilide substrates at acidic pHs | Aspergillus fumigatus NCPF 7367 | ? | - |
? | |
| Phe-Pro-Ala-p-nitroanilide + H2O | SedB, SedC, and SedD | Aspergillus fumigatus | Phe-Pro-Ala + p-nitroaniline | - |
? | |
| Phe-Pro-Ala-p-nitroanilide + H2O | SedB, SedC, and SedD | Aspergillus fumigatus NCPF 7367 | Phe-Pro-Ala + p-nitroaniline | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 479000, mature sedA, sequence analysis. x * 430000, mature sedB, sequence analysis. x * 406000, mature sedD, sequence analysis. x * 55000, native sedA, SDS-PAGE. x * 60000-90000, native sedB, SDS-PAGE. x * 65000, native sedC, SDS-PAGE. x * 70000-100000, native sedD, SDS-PAGE. x * 46000, sedA and sedB after deglycosylation, SDS-PAGE. x * 48000, sedC and sedD after deglycosylation, SDS-PAGE | Aspergillus fumigatus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SedA | - |
Aspergillus fumigatus |
| SedB | - |
Aspergillus fumigatus |
| SedC | - |
Aspergillus fumigatus |
| SedD | - |
Aspergillus fumigatus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 35 | - |
Phe-Pro-Ala-p-nitroanilide | - |
Aspergillus fumigatus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
SedB and SedC have an optimum at pH 6.0, while SedD has an optimum peak at pH 5.0 | Aspergillus fumigatus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 1.5 | 7 | SedB and SedC are active between pH 3.0 and 7.0, while SedD is active between pH 1.5 and 6.0 | Aspergillus fumigatus |
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Release 2023.1 (January 2023)
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